Product Covid-19 Colloidal Gold Immunofluorescence Chemiluminescence PCR
Home /

Immunofluorescence

/follicle stimulating hormone

follicle stimulating hormone

It is mainly used to quantitatively detect the content of follicle stimulating hormone (FSH) in human serum, plasma and whole blood in vitro. 
  • Brand:

    Norman
  • Payment:

    T/T
  • Specification:

    1/25 Tests
Share To : f t in y p
  • Product Detail

Generic name: Follicle Stimulating Hormone Detection Kit (Dry Fluorescence Immunoluminescence Method)
【Packing specifications】
10 servings/box, 2×10 servings/box, 3×10 servings/box, 4×10 servings/box, 5×10 servings/box, 6×10 servings/box,
15 servings/box, 2×15 servings/box, 3×15 servings/box, 4×15 servings/box, 5×15 servings/box, 6×15 servings/box,
20 servings/box, 2×20 servings/box, 3×20 servings/box, 4×20 servings/box, 5×20 servings/box, 6×20 servings/box,
25 servings/box, 2×25 servings/box, 3×25 servings/box, 4×25 servings/box, 5×25 servings/box, 6×25 servings/box,
30 servings/box, 2×30 servings/box, 3×30 servings/box, 4×30 servings/box, 5×30 servings/box, 6×30 servings/box,
35 servings/box, 2×35 servings/box, 3×35 servings/box, 4×35 servings/box, 5×35 servings/box, 6×35 servings/box,
40 servings/box, 2×40 servings/box, 3×40 servings/box, 4×40 servings/box, 5×40 servings/box, 6×40 servings/box,
50 servings/box, 2×50 servings/box.
【expected usage】
It is mainly used to quantitatively detect the content of Follicle Stimulating Hormone (FSH) in human serum, plasma, and whole blood in vitro.
Follicle Stimulating Hormone (FSH) is a hormone secreted by basophils in the anterior pituitary gland. It is formed by the covalent cross-linking of α and β subunits. The difference in β subunits results in immune and physiological specificity [1 ]. In women, FSH and LH jointly stimulate the secretion and ovulation of estrogen, which can stimulate the growth and maturation of follicles, and play a role in the hypothalamic-pituitary-ovarian regulatory loop. FSH in men is a necessary condition for normal sperm maturation, which can induce the development of spermatogonia and stimulate sperm production.
The combined detection of FSH and LH can be used for the detection of abnormal menstrual cycle, fertility and puberty development. Low levels of FSH and LH may indicate secondary sexual dysfunction, pituitary failure, azoospermia, etc. Abnormally elevated levels of FSH and LH may indicate gonadal failure, primary reproductive dysfunction, Turner syndrome, Klinefelter syndrome, etc.
Commonly used detection methods in clinical laboratories mainly include colloidal gold method, dry fluorescent immunoluminescence method, enzyme-linked immunoassay, chemiluminescence method, etc.
【Principle of Inspection】
This reagent uses immunofluorescence double antibody sandwich method to quantitatively detect the content of follicle-stimulating hormone in the sample. Drop the sample to be tested into the sample hole of the test card. Under the action of chromatography, the sample and the binding pad are coated with the fluorescent latex reaction of the follicle-stimulating hormone monoclonal antibody I, and the reaction complex moves along the nitrocellulose membrane. The front diffusion is captured by the FSH monoclonal antibody II coated on the nitrocellulose membrane detection line. The more FSH in the sample, the more complexes accumulated on the detection line. The intensity of the fluorescent antibody signal reflects the number of FSH captured. The concentration of FSH in the sample can be determined by the matching applicable instrument.
【Main components】
Test card: It consists of a test strip shell and a test strip. The main components on the test strip are: sample pad and binding pad (containing fluorescent latex particles coated with FSH monoclonal antibody I (mouse) (coated antibody) 0.5 μg/person), nitrocellulose membrane (the detection area is coated with FSH monoclonal antibody II (mouse) (0.5μg/person)), and the quality control area is coated with goat anti-mouse IgG antibody (0.5 μg/person) Copies), absorbent paper, PVC bottom plate.
Follicle Stimulating Hormone Sample Diluent: It is composed of phosphate (10mmol/L) and surfactant (Tween-20, 0.2%), pH=7.4±0.2.
In addition, it is also equipped with a calibration card, a dilution pipette (optional) and a sample tank (optional).
※The components in the kits of different batch numbers are not interchangeable.
Equipment, reagents and consumables that need to be purchased separately:
Reagent card incubator
[Storage conditions and validity period]
The product is stored at 4-30°C, and the validity period is 24 months.
After the aluminum foil bag is opened, the validity period is 1 hour.
The sample diluent is stored at 2-8°C after opening the bottle, and the validity period is 12 months; after opening the bottle, it is stored at room temperature and the validity period is 1 month.
【Applicable instrument】
    Immunofluorescence quantitative analyzer (NRM-FI-1000), fluorescence immunoassay analyzer (FI-1200/FI-1200A/FI-1200B), automatic fluorescence immunoassay analyzer (FI-2000A/FI-2000B/FI-3000A/ FI-3000B), the automatic dry fluorescence immunoassay analyzer (AFS4000) produced by Guangzhou Lanbo Biotechnology Co., Ltd.
【Sample requirements】
1. It is recommended to use EDTA for anticoagulation for whole blood samples, and it is recommended to complete the test within 4 hours for samples. If the test cannot be completed within 4 hours, the sample should be placed at 2-8°C and the test should be completed within 24 hours. Freezing of whole blood samples is prohibited.
2. It is recommended to use EDTA for anticoagulation of plasma samples. The samples are recommended to be tested within 4 hours. If they cannot be used immediately, it is recommended to store them at 2-8°C and complete the test within 24 hours. If the test cannot be performed within 24 hours, the sample must be frozen at -20°C, and the validity period is 30 days.
3. Serum is collected by ordinary serum tube, fast serum tube or inert separation gel coagulation tube. It is recommended that the sample be tested within 4 hours. If it cannot be used immediately, it is recommended to store it at 2-8°C and complete the test within 24 hours. If the test cannot be performed within 24 hours, the sample must be frozen at -20°C, and the validity period is 30 days.
4. The samples must be returned to room temperature (15-30°C) before testing. The frozen plasma or serum samples must be completely thawed, rewarmed, and mixed before use. Avoid repeated freezing and thawing; do not freeze whole blood samples

Leave A Message
If you are interested in our products and want to know more details,please leave a message here,we will reply you as soon as we can.
Leave A Message
Leave A Message
If you are interested in our products and want to know more details,please leave a message here,we will reply you as soon as we can.

Home

Products

about

contact