Principle of chromatography
The principle of chromatography is a separation technique established on utilizing the physical property difference of various components in the mixture (e.g., adsorption, molecular shape and size, molecular polarity, molecular affinity, distribution coefficient, etc.). Chromatography system is composed of stationary phase and mobile phase. Stationary phase is solid substance or immobilized content on solid material. The mobile phase is flowing substance, such as water or various solvents.
Chromatographic process: When the mixture to be separated runs with the mobile phase through the stationary phase, due to the differences in physical and chemical properties of various components in the mixture, the components that have weak interaction with the stationary phase incur less blocking effect when run with the mobile phase, so they move forward faster; those components with strong interaction with the stationary phase move forward more slowly. Therefore the separation of various components in the mixture is fulfilled.
Immuno-chromatography technique is an emerging immunoassay technology based on the chromatographic techniques and antigen - antibody specific immune response.
Immuno-chromatography technique takes strip fiber chromatographic material fixed with the testing line and control line as stationary phase, takes the test solution as the mobile phase, move the analytes on the chromatographic strip by capillary action. The analytes cause specific immunoreaction at T-line. The free material causes immunoreaction at C-line.
Principle schematic diagram of immune chromatography